| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/2000/01/rdf-schema#comment | "The hadBC and hadI genes from Clostridium difficile were functionally expressed in Escherichia coli and shown to encode the novel 2-hydroxyisocaproyl-CoA dehydratase HadBC and its activator HadI. The activated enzyme catalyses the dehydration of (R)-2-hydroxyisocaproyl-CoA to isocaprenoyl-CoA in the pathway of leucine fermentation. The extremely oxygen-sensitive homodimeric activator as well as the heterodimeric dehydratase, contain iron and inorganic sulfur; besides varying amounts of zinc, other metal ions, particularly molybdenum, were not detected in the dehydratase. The reduced activator transfers one electron to the dehydratase concomitant with hydrolysis of ATP, a process similar to that observed with the unrelated nitrogenase. The thus activated dehydratase was separated from the activator and ATP; it catalyzed about 10(4) dehydration turnovers until the enzyme became inactive. Adding activator, ATP, MgCl(2), dithionite and dithioerythritol reactivated the enzyme. This is the first demonstration with a 2-hydroxyacyl-CoA dehydratase that the catalytic electron is recycled after each turnover. In agreement with this observation, only substoichiometric amounts of activator (dehydratase/activator = 10 mol/mol) were required to generate full activity."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2004.04498.x"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2004.04498.x"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Buckel W."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Buckel W."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Kim J."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Kim J."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Darley D."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/author | "Darley D."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/pages | "550-561"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/pages | "550-561"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/title | "2-hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/title | "2-hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile."xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15654892 |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15654892 |
| http://purl.uniprot.org/citations/15654892 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15654892 |