| http://purl.uniprot.org/citations/15654973 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15654973 | http://www.w3.org/2000/01/rdf-schema#comment | "Store-operated calcium entry depicts the movement of extracellular Ca2+ into cells through plasma membrane Ca2+ channels activated by depletion of intracellular Ca2+ stores. The members of the canonical subfamily of transient receptor potential channels (TRPC) have been implicated as the molecular bases for store-operated channels (SOC). Here we investigate the role of phospholipase C (PLC) in regulation of native SOC and the expression of endogenous TRPC in human epidermal keratinocytes. Calcium entry in response to store depletion with thapsigargin was reversibly blocked by 2-aminoethoxydiphenyl borane, an effective SOC inhibitor, and suppressed by the diacylglycerol analoge, 1-oleoyl-2-acetyl-sn-glycerol. Inhibition of PLC with U73122 or transfection of a PLCgamma1 antisense cDNA construct completely blocked SOC activity, indicating a requirement for PLC, especially PLCgamma1, in the activation of SOC. RT-PCR and immunoblotting analyses showed that TRPC1, TRPC3, TRPC4, TRPC5, and TRPC6 are expressed in keratinocytes. Knockdown of the level of endogenous TRPC1 or TRPC4 inhibited store-operated calcium entry, indicating they are part of the native SOC. Co-immunoprecipitation studies demonstrated that TRPC1, but not TRPC4, interacts with PLCgamma1 and the inositol 1,4,5-trisphosphate receptor (IP3R). The association of TRPC1 with PLCgamma1 and IP3R decreased in keratinocytes with higher intracellular Ca2+, coinciding with a downregulation in SOC activity. Our results indicate that the activation of SOC in keratinocytes depends, at least partly, on the interaction of TRPC with PLCgamma1 and IP3R."xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.0022-202x.2004.23544.x"xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/author | "Chang W."xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/author | "Bikle D.D."xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/author | "Tu C.L."xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/name | "J Invest Dermatol"xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/pages | "187-197"xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/title | "Phospholipase cgamma1 is required for activation of store-operated channels in human keratinocytes."xsd:string |
| http://purl.uniprot.org/citations/15654973 | http://purl.uniprot.org/core/volume | "124"xsd:string |
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