| http://purl.uniprot.org/citations/15657399 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15657399 | http://www.w3.org/2000/01/rdf-schema#comment | "Contributions of actin-related proteins (Arp) 2 and 3 nucleotide state to Arp2/3 complex function were tested using nucleotide-binding pocket (NBP) mutants in Saccharomyces cerevisiae. ATP binding by Arp2 and Arp3 was required for full Arp2/3 complex nucleation activity in vitro. Analysis of actin dynamics and endocytosis in mutants demonstrated that nucleotide-bound Arp3 is particularly important for Arp2/3 complex function in vivo. Severity of endocytic defects did not correlate with effects on in vitro nucleation activity, suggesting that a critical Arp2/3 complex function during endocytosis may be structural rather than catalytic. A separate class of Arp2 and Arp3 NBP mutants suppressed phenotypes of mutants defective for actin nucleation. An Arp2 suppressor mutant increased Arp2/3 nucleation activity. Electron microscopy of Arp2/3 complex containing this Arp2 suppressor identified a structural change that also occurs upon Arp2/3 activation by nucleation promoting factors. These data demonstrate the importance of Arp2 and Arp3 nucleotide binding for nucleating activity, and Arp3 nucleotide binding for maintenance of cortical actin cytoskeleton cytoarchitecture."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.200408177"xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Martin A.C."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Rouiller I."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Drubin D.G."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Hanein D."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Volkmann N."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Xu X.P."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Welch M."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Kaksonen M."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/author | "Belmont L."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/pages | "315-328"xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/title | "Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function."xsd:string |
| http://purl.uniprot.org/citations/15657399 | http://purl.uniprot.org/core/volume | "168"xsd:string |
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