| http://purl.uniprot.org/citations/15663743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15663743 | http://www.w3.org/2000/01/rdf-schema#comment | "The characterization of genetic risk factors for complex diseases located on chromosome-6 frequently requires human leucocyte antigen (HLA) genotyping of large patient cohorts. Currently available methods do not support high-throughput HLA typing beyond the major allele group level. We, thus, developed a high-throughput approach for the HLA-DQB1 and HLA-DRB1 loci that is based on Pyrosequencing. Pyrosequencing offers a higher degree of automation than direct sequencing or oligotyping. Using a dispensation order optimized for the particular HLA locus, rapid group typing and fine resolution can be achieved. We implemented the method for two important HLA loci--DQB1 and DRB1. The HLA-DQB1 typing method comprises the following steps: splitting the potential alleles after a generic polymerase chain reaction (PCR) amplification into groups with a first Pyrosequencing reaction and resolving the split allele groups by means of five further Pyrosequencing reactions. The HLA-DR gene family is known to be the most polymorphic one in the HLA class-II region because of a large number of DRB1 alleles. Because of this complex nature, HLA-DRB1 typing was performed by means of a combination of sequence-specific PCR typing and Pyrosequencing. HLA-DQB1 typing and HLA-DRB1 typing were performed successfully by using standard DNA samples with the help of known HLA genotypes and in a blind study by using the samples from the Deutscher Zell Austausch 2002 and 2003. The approach was optimized and was practically tested for genotyping in disease association studies. Our well-elaborated Pyrosequencing-based protocols offer a new alternative to the existing HLA class-II typing methods and represent a convenient and economic solution, a unique combination of high accuracy with high-sample throughput."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1399-0039.2005.00345.x"xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Hampe J."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Nurnberg P."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Toliat M.R."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Nagy M."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Valentonyte R."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Jenisch S."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/author | "Entz P."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/name | "Tissue Antigens"xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/pages | "67-80"xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/title | "New strategies for efficient typing of HLA class-II loci DQB1 and DRB1 by using Pyrosequencing."xsd:string |
| http://purl.uniprot.org/citations/15663743 | http://purl.uniprot.org/core/volume | "65"xsd:string |
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