| http://purl.uniprot.org/citations/15677454 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15677454 | http://www.w3.org/2000/01/rdf-schema#comment | "Tal1/SCL is a basic helix-loop-helix transcription factor critical for normal hematopoiesis. To understand the mechanisms underlying transcriptional regulation by Tal1/SCL, we combined an in vitro DNA binding strategy and an in vivo chromatin immunoprecipitation analysis to search for Tal1/SCL target regions in K562 erythroleukemia cells. A 0.4-kb genomic DNA clone containing two Tal1/SCL binding E-boxes and GATA- and SATB1-binding motifs (EEGS) was identified that localized to the pericentromeric region with high homology to satellite 2 DNA. Pericentric DNA is related to heterochromatin and gene inactivation. We found that Tal1/SCL could complex with the histone H3 lysine 9 (H3K9)-specific methyltransferase Suv39H1. Binding of Tal1/SCL to EEGS chromatin correlated with hypermethylation of H3K9 and the association of heterochromatin protein HP1 to this region. In Rep4 reporter gene assays, EEGS affected repression in a manner dependent on the expression level of Tal1/SCL that was accompanied by increased H3K9 methylation in chromatin associated with EEGS and a linked promoter. A specific histone deacetylase inhibitor, trichostatin A, relieved Tal1/SCL-mediated repression by EEGS. In addition, SATB1 bound EEGS chromatin and promoted Tal1/SCL EEGS-dependent repression. We expand the list of potential interacting partners for Tal1/SCL by demonstrating direct associations of Tal1/SCL with SATB1 and with Suv39H1. These results reveal a novel mechanism of action for Tal1/SCL and implicate heterochromatin-like silencing via a cis-acting binding motif for transcriptional repression."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m412721200"xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Huang S."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Yu X."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Wen J."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Pack S.D."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Noguchi C.T."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/author | "Brandt S.J."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/pages | "12956-12966"xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/title | "Tal1/SCL binding to pericentromeric DNA represses transcription."xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15677454 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15677454 |
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