| http://purl.uniprot.org/citations/15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15679100 | http://www.w3.org/2000/01/rdf-schema#comment | "Centaurin alpha-1 is a high-affinity PtdIns(3,4,5)P3-binding protein enriched in brain. Sequence analysis indicates centaurin alpha-1 contains two pleckstrin homology domains, ankyrin repeats and an Arf GAP homology domain, placing it in the AZAP family of phosphoinositide-regulated Arf GAPs. Other members of this family are involved in actin cytoskeletal and focal adhesion organization. Recently, it was reported that centaurin alpha-1 expression diminishes cortical actin and decreases Arf6GTP levels consistent with it functioning as an Arf6 GAP in vivo. In the current report, we show that centaurin alpha-1 binds Arfs in vitro and colocalizes with Arf6 and Arf5 in vivo, further supporting an interaction with Arfs. Centaurin alpha-1 expression produces dramatic effects on the actin cytoskeleton, decreasing stress fibers, diminishing cortical actin, and enhancing membrane ruffles and filopodia. Expression of centaurin alpha-1 also enhances cell spreading and disrupts focal adhesion protein localization. The effects of centaurin alpha-1 on stress fibers and cell spreading are reminiscent of those of Arf6GTP. Consistent with this, we show that many of the centaurin alpha-1-induced effects on the actin cytoskeleton and actin-dependent activities do not require GAP activity. Thus, centaurin alpha-1 likely functions via both GAP-dependent and GAP-independent mechanisms to regulate the actin cytoskeleton. Furthermore, we demonstrate that in vitro, centaurin alpha-1 binds F-actin directly, with actin binding activity localized to the PtdIns(3,4,5)P3-binding PH domain. Our data suggest that centaurin alpha-1 may be a component of the neuronal PI 3-kinase cascade that leads to regulation of the neuronal actin cytoskeleton."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.org/dc/terms/identifier | "doi:10.1078/0171-9335-00416"xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Hammond J."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Chapman C."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Howell A."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Kearns B."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Thacker E."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/author | "Theibert A."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/name | "Eur J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/pages | "541-554"xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/title | "The arf6 GAP centaurin alpha-1 is a neuronal actin-binding protein which also functions via GAP-independent activity to regulate the actin cytoskeleton."xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://purl.uniprot.org/core/volume | "83"xsd:string |
| http://purl.uniprot.org/citations/15679100 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15679100 |
| http://purl.uniprot.org/citations/15679100 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15679100 |
| http://purl.uniprot.org/uniprot/#_A8K3A2-mappedCitation-15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/#_B4DUH0-mappedCitation-15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/#_B4DUZ7-mappedCitation-15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/#_B3KWF6-mappedCitation-15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/#_O75689-mappedCitation-15679100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/O75689 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/B4DUH0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/B3KWF6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15679100 |
| http://purl.uniprot.org/uniprot/B4DUZ7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15679100 |