| http://purl.uniprot.org/citations/15687495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15687495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15687495 | http://www.w3.org/2000/01/rdf-schema#comment | "LSL is a lectin produced by the parasitic mushroom Laetiporus sulphureus, which exhibits hemolytic and hemagglutinating activities. Here, we report the crystal structure of LSL refined to 2.6-A resolution determined by the single isomorphous replacement method with the anomalous scatter (SIRAS) signal of a platinum derivative. The structure reveals that LSL is hexameric, which was also shown by analytical ultracentrifugation. The monomeric protein (35 kDa) consists of two distinct modules: an N-terminal lectin module and a pore-forming module. The lectin module has a beta-trefoil scaffold that bears structural similarities to those present in toxins known to interact with galactose-related carbohydrates such as the hemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum, abrin, and ricin. On the other hand, the C-terminal pore-forming module (composed of domains 2 and 3) exhibits three-dimensional structural resemblances with domains 3 and 4 of the beta-pore-forming toxin aerolysin from the Gram-negative bacterium Aeromonas hydrophila, and domains 2 and 3 from the epsilon-toxin from Clostridium perfringens. This finding reveals the existence of common structural elements within the aerolysin-like family of toxins that could be directly involved in membrane-pore formation. The crystal structures of the complexes of LSL with lactose and N-acetyllactosamine reveal two dissacharide-binding sites per subunit and permits the identification of critical residues involved in sugar binding."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M413933200"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m413933200"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Tateno H."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Tateno H."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Hermoso J.A."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Hermoso J.A."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Goldstein I.J."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Goldstein I.J."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Mancheno J.M."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Mancheno J.M."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Martinez-Ripoll M."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/author | "Martinez-Ripoll M."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/pages | "17251-17259"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/pages | "17251-17259"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/title | "Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/title | "Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars."xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15687495 | http://purl.uniprot.org/core/volume | "280"xsd:string |