| http://purl.uniprot.org/citations/1569083 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1569083 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1569083 | http://www.w3.org/2000/01/rdf-schema#comment | "CCAAT-binding factors A (CBF-A) and B (CBF-B) are two subunits of the heteromeric CCAAT-binding factor. Portions of CBF-A and CBF-B have a high degree of amino acid sequence identity to segments of the HAP3 and HAP2 subunits of a yeast multimeric transcription factor. We show here that the subunits of CBF interact with each other in the absence of DNA binding. This interaction was revealed by cross-linking and coimmunoprecipitation studies. Both the DNA binding and subunit interaction functions of CBF-B have been examined by mutational analysis. A segment of 83 amino acids from residues 252 to 334, which corresponds to the evolutionarily conserved portion of CBF-B, is necessary and sufficient for CBF-A-dependent DNA binding. Carboxyl-terminal deletions of this segment (or mutations in arginine residues in this carboxyl-terminal part) abolish DNA binding, but do not alter subunit interactions between CBF-A and CBF-B. Mutations in hydrophobic amino acids within the amino-terminal part of the evolutionarily conserved sequence at positions 252-334 result in loss of both DNA binding and subunit interaction activities. Our results indicate that the evolutionarily conserved segment of CBF-B contains both DNA-binding and subunit interaction domains and that the integrity of both domains is essential for DNA binding."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42440-4"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42440-4"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/author | "de Crombrugghe B."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/author | "de Crombrugghe B."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/author | "Maity S.N."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/author | "Maity S.N."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/pages | "8286-8292"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/pages | "8286-8292"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/title | "Biochemical analysis of the B subunit of the heteromeric CCAAT-binding factor. A DNA-binding domain and a subunit interaction domain are specified by two separate segments."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/title | "Biochemical analysis of the B subunit of the heteromeric CCAAT-binding factor. A DNA-binding domain and a subunit interaction domain are specified by two separate segments."xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/volume | "267"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://purl.uniprot.org/core/volume | "267"xsd:string |
| http://purl.uniprot.org/citations/1569083 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1569083 |
| http://purl.uniprot.org/citations/1569083 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1569083 |
| http://purl.uniprot.org/citations/1569083 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1569083 |
| http://purl.uniprot.org/citations/1569083 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1569083 |
| http://purl.uniprot.org/uniprot/P18576 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/1569083 |
| http://purl.uniprot.org/uniprot/#_P18576-citation-1569083 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1569083 |