| http://purl.uniprot.org/citations/15694127 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15694127 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15694127 | http://www.w3.org/2000/01/rdf-schema#comment | "Agrin is a key organizer for postsynaptic differentiation at the neuromuscular junction (NMJ). This activity requires the binding of agrin to the synaptic basal lamina via its N-terminal (NtA) domain. It has been suggested that this binding is mediated by conserved amino acids in the gamma 1 chain of laminin. Here, we report the crystal structure of chicken NtA expressed in eukaryotic HEK293 cells. In contrast to the previously published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA domain expressed in E. coli, the new data show that the N-terminal tail region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the disulfide bridge between Cys2 and Cys74 was also present. In addition, we show that the binding of NtA requires the gamma 1 chain of laminin and is not greatly affected by the composition of beta chains. These results confirm a model of the NtA-laminin complex where conserved amino acids in the gamma 1 chain are prerequisite for the binding to agrin and they further emphasize that the source of protein can be critical in structure determination."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.matbio.2004.11.003"xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.matbio.2004.11.003"xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Sasaki T."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Sasaki T."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Engel J."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Engel J."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Eble J.A."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Eble J.A."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Stetefeld J."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Stetefeld J."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Mascarenhas J.B."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Mascarenhas J.B."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Rueegg M.A."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/author | "Rueegg M.A."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/name | "Matrix Biol."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/name | "Matrix Biol."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/pages | "507-513"xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/pages | "507-513"xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/title | "Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells."xsd:string |
| http://purl.uniprot.org/citations/15694127 | http://purl.uniprot.org/core/title | "Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells."xsd:string |