| http://purl.uniprot.org/citations/15694994 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15694994 | http://www.w3.org/2000/01/rdf-schema#comment | "Increasing evidence has implicated that insulin-like growth factors (IGFs), polypeptides structurally related to proinsulin, are involved in the function and development of the immune system. To probe the relevance of IGF binding protein 2 (IGFBP-2) in T-cell activation and proliferation, we studied the role of IGFBP-2 in anti-CD3 monoclonal antibody (mAb)-activated peripheral blood mononuclear cells (PBMCs). Secretion of IGF-I, IGF-II, and IGFBP-2 by PBMCs from healthy adult donors was determined by radioimmunoassays (RIAs). The PBMC proliferative response after stimulation with anti-CD3 mAb and exposure to increasing concentrations of IGF-I, IGF-II, IGFBP-2, and anti-IGFBP-2 were determined by bromodeoxyuridine enzyme-linked immunosorbent assay. Observations were tested for significance by paired t-tests. We demonstrate an increase in IGFBP-2 secretion associated with both activation of PBMC by anti-CD3 mAb and increasing cell density. Incubation with exogenous IGFBP-2 increased the proliferation of PBMCs, whereas anti-IGFBP-2 had an antiproliferative effect on PBMCs that was reversed by simultaneous exposure to IGFBP-2. The stimulatory activity of IGFBP-2 (1-10 ng/ml) on anti-CD3 mAb-activated PBMCs was similar to that of IGF-I and IGF-II (1-100 ng/ml), with the mean increase in PBMC proliferative response ranging between 150% and 160% for IGFBP-2 (p = 0.03), 150% and 170% for IGF-I (p < 0.01), 133%-161% for IGF-II (p < 0.01), and 157% and 175% for IGF-I + IGF-II (p < 0.01). Thus, our data strongly suggest a role for IGFBP-2 as a local growth factor contributing to the proliferation and activation of mononuclear cells."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.humimm.2004.10.014"xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/author | "Elmlinger M.W."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/author | "Foell J."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/author | "Hettmer S."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/author | "Dannecker G.E."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/author | "Dannecker L."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/name | "Hum Immunol"xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/pages | "95-103"xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/title | "Effects of insulin-like growth factors and insulin-like growth factor binding protein-2 on the in vitro proliferation of peripheral blood mononuclear cells."xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://purl.uniprot.org/core/volume | "66"xsd:string |
| http://purl.uniprot.org/citations/15694994 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15694994 |
| http://purl.uniprot.org/citations/15694994 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15694994 |
| http://purl.uniprot.org/uniprot/P05019#attribution-5CE022684FBC35BFD4F9570DD10E048B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15694994 |
| http://purl.uniprot.org/uniprot/P18065#attribution-5CE022684FBC35BFD4F9570DD10E048B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15694994 |
| http://purl.uniprot.org/uniprot/P01344#attribution-5CE022684FBC35BFD4F9570DD10E048B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15694994 |