| http://purl.uniprot.org/citations/15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15696577 | http://www.w3.org/2000/01/rdf-schema#comment | "Poly(ADP-ribose) polymerase-1 (PARP-1) plays a critical role in endothelial cell dysfunction associated with various pathophysiological conditions. To elucidate PARP-1 pathways involved in endothelial cell dysfunction, it is essential to establish "in vitro" experimental models using isolated endothelial cells. So far, two approaches have been used: primary endothelial cells from PARP-1-/-mice which have a limited life-span, being a major handicap if large quantities of cells are required; and pharmacological inhibition of PARP in PARP-1+/+ endothelial cell lines, which is not specific for PARP-1 and would have biological effects different that genetic inhibition. To overcome these limitations, we have established an immortalized PARP-1-/-endothelial cell line (HYKO6) by transfection of primary cells with a plasmid containing the SV40 genome and selected on the basis of morphological and phenotypical features. The HYKO6 cell line exhibited endothelial characteristics, such as constitutive expression of CD105, CD31, ICAM-2, VCAM-1, and von Willebrand factor and formation of capillary-like structures (CLS) on Matrigel surface. However, expression of ICAM-1 antigen is lost in the HYKO6 cells. After TNF-alpha treatment, HYKO6 cells exhibited increased expression of E-selectin and VCAM-1. Likewise, NF-kappaB-dependent transcriptional activation was increased in the HYKO6 cell line in response to TNF-alpha at a level similar to that found for primary PARP-1-/-cells. This cell line should provide, for the first time, a valuable tool to study PARP-1 pathways in endothelial cell dysfunction."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.20385"xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Ramirez P."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Menissier-de Murcia J."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "de Murcia G."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Yelamos J."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Suarez E."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Carrillo A."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Parrilla P."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Monreal Y."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/author | "Alvarez-Vallina L."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/pages | "1163-1174"xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/title | "Establishment of an immortalized PARP-1-/- murine endothelial cell line: a new tool to study PARP-1 mediated endothelial cell dysfunction."xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://purl.uniprot.org/core/volume | "94"xsd:string |
| http://purl.uniprot.org/citations/15696577 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15696577 |
| http://purl.uniprot.org/citations/15696577 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15696577 |
| http://purl.uniprot.org/uniprot/#_P11103-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |
| http://purl.uniprot.org/uniprot/#_Q3U868-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |
| http://purl.uniprot.org/uniprot/#_Q3TX36-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |
| http://purl.uniprot.org/uniprot/#_Q921K2-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |
| http://purl.uniprot.org/uniprot/#_Q3UJ03-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |
| http://purl.uniprot.org/uniprot/#_P97340-mappedCitation-15696577 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15696577 |