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| http://purl.uniprot.org/citations/15707389 | http://www.w3.org/2000/01/rdf-schema#comment | "PKCzeta (protein kinase Czeta) is a serine/threonine protein kinase controlled by insulin, various growth factors and phosphoinositide 3-kinase. It has been implicated in controlling glucose transport in response to insulin by the translocation of GLUT4-(glucose transporter 4) containing vesicles to the plasma membrane in stimulated cells. How PKCzeta modulates GLUT4 vesicle trafficking remains unknown. A yeast two-hybrid screen using full-length human PKCzeta identified 80K-H protein as an interactor with PKCzeta. GST (glutathione S-transferase) pull-down assays with GST-tagged 80K-H constructs confirmed the interaction and showed that the N-terminal portion of 80K-H was not required for the interaction. Immunoprecipitates of endogenous PKCzeta from Cho cells, 3T3-L1 adipocytes or L6 myotubes contained endogenous 80K-H, demonstrating a physiological interaction. Insulin stimulation enhanced the association 3-5-fold. Immunoprecipitates of endogenous 80K-H contained endogenous munc18c and immunoprecipitates of endogenous munc18c contained endogenous PKCzeta, with insulin markedly increasing the amount of co-immunoprecipitated protein in each case. These results show that insulin triggers interactions in vivo between PKCzeta, 80K-H and munc18c. Overexpression of 80K-H constructs mimicked the action of insulin in stimulating both glucose uptake and translocation of Myc-tagged GLUT4 in Cho cells, with the level of effect proportional to the ability of the constructs to associate with munc18c. These results identify 80K-H as a new player involved in GLUT4 vesicle transport and identify a link between a kinase involved in the insulin signalling cascade, PKCzeta, and a known component of the GLUT4 vesicle trafficking pathway, munc18c. The results suggest a model whereby insulin triggers the formation of a PKCzeta-80K-H-munc18c complex that enhances GLUT4 translocation to the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20041845"xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/author | "Hodgkinson C.P."xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/author | "Sale G.J."xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/author | "Mander A."xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/pages | "785-793"xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/title | "Identification of 80K-H as a protein involved in GLUT4 vesicle trafficking."xsd:string |
| http://purl.uniprot.org/citations/15707389 | http://purl.uniprot.org/core/volume | "388"xsd:string |
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