| http://purl.uniprot.org/citations/15713649 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15713649 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15713649 | http://www.w3.org/2000/01/rdf-schema#comment | "CENP-H is a constitutive centromere component that localizes to the centromere throughout the cell cycle. Because CENP-H is colocalized with CENP-A and CENP-C, it is thought to be an inner centromere protein. We previously generated a conditional loss-of-function mutant of CENP-H and showed that CENP-H is required for targeting of CENP-C to the centromere in chicken DT40 cells. In the present study, we used this mutant to identify the functional region of chicken CENP-H necessary for centromere targeting and cell viability. This region was found by yeast two-hybrid analysis to interact with Hec1, which is a member of the Nuf2 complex that transiently localizes to the centromere during mitosis. Coimmunoprecipitation experiments revealed that CENP-H interacts with the Nuf2 complex in chicken DT40 cells. Photobleaching experiments showed that both Hec1 and CENP-H form stable associations with the centromeres during mitosis, suggesting that Hec1 acts as a structural component of centromeres during mitosis. On the basis of these results and previously published data, we propose that the Nuf2 complex functions as a connector between the inner and outer kinetochores."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.5.1958-1970.2005"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.5.1958-1970.2005"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Mikami Y."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Mikami Y."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Kimura H."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Kimura H."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Hori T."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Hori T."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Fukagawa T."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/author | "Fukagawa T."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/pages | "1958-1970"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/pages | "1958-1970"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/title | "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/title | "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/15713649 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15713649 |
| http://purl.uniprot.org/citations/15713649 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15713649 |