| http://purl.uniprot.org/citations/15743815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15743815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15743815 | http://www.w3.org/2000/01/rdf-schema#comment | "Most DNA polymerases incorporate nucleotides opposite template 7,8-dihydro-8-oxoguanine (8-oxoG) lesions with reduced efficiency and accuracy. DNA polymerase (Pol) eta, which catalyzes the error-free replication of template thymine-thymine (TT) dimers, has the unique ability to accurately and efficiently incorporate nucleotides opposite 8-oxoG templates. Here we have used pre-steady-state kinetics to examine the mechanisms of correct and incorrect nucleotide incorporation opposite G and 8-oxoG by Saccharomyces cerevisiae Pol eta. We found that Pol eta binds the incoming correct dCTP opposite both G and 8-oxoG with similar affinities, and it incorporates the correct nucleotide bound opposite both G and 8-oxoG with similar rates. While Pol eta incorporates an incorrect A opposite 8-oxoG with lower efficiency than it incorporates a correct C, it does incorporate A more efficiently opposite 8-oxoG than opposite G. This is mainly due to greater binding affinity for the incorrect incoming dATP opposite 8-oxoG. Overall, these results show that Pol eta replicates through 8-oxoG without any barriers introduced by the presence of the lesion."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.6.2169-2176.2005"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.6.2169-2176.2005"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/author | "Carlson K.D."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/author | "Carlson K.D."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/author | "Washington M.T."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/author | "Washington M.T."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/pages | "2169-2176"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/pages | "2169-2176"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/title | "Mechanism of efficient and accurate nucleotide incorporation opposite 7,8-dihydro-8-oxoguanine by Saccharomyces cerevisiae DNA polymerase eta."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/title | "Mechanism of efficient and accurate nucleotide incorporation opposite 7,8-dihydro-8-oxoguanine by Saccharomyces cerevisiae DNA polymerase eta."xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/15743815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15743815 |
| http://purl.uniprot.org/citations/15743815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15743815 |
| http://purl.uniprot.org/citations/15743815 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15743815 |
| http://purl.uniprot.org/citations/15743815 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15743815 |
| http://purl.uniprot.org/uniprot/Q04049 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/15743815 |
| http://purl.uniprot.org/uniprot/Q04049#attribution-C7D985DDA7BE85D09D5D388EF6A3CC99 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15743815 |