| http://purl.uniprot.org/citations/15746095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15746095 | http://www.w3.org/2000/01/rdf-schema#comment | "The heterotrimeric G protein G(12) has been implicated in such cellular regulatory processes as cytoskeletal rearrangement, cell-cell adhesion, and oncogenic transformation. Although the activated alpha-subunit of G(12) has been shown to interact directly with a number of protein effectors, the roles of many of these protein-protein interactions in G(12)-mediated cell physiology are poorly understood. To begin dissecting the specific cellular pathways engaged upon G(12) activation, we produced a series of substitution mutants in the regions of Galpha(12) predicted to play a role in effector binding. Here we report the identification and characterization of an altered form of Galpha(12) that is functionally uncoupled from signaling through the monomeric G protein Rho, a protein known to propagate several Galpha(12)-mediated signals. This mutant of Galpha(12) fails to bind the Rho-specific guanine nucleotide exchange factors p115RhoGEF and LARG (leukemia-associated RhoGEF), fails to stimulate Rho-dependent transcriptional activation, and fails to trigger activation of RhoA and the Rho-mediated cellular responses of cell rounding and c-jun N-terminal kinase activation. Importantly, this mutant of Galpha(12) retains coupling to the effector protein E-cadherin, as evidenced by its ability both to bind E-cadherin in vitro and to disrupt E-cadherin-mediated cell-cell adhesion. Furthermore, this mutant retains the ability to trigger beta-catenin release from the cytoplasmic domain of cadherin. This identification of a variant of Galpha(12) that is selectively uncoupled from one signaling pathway while retaining signaling capacity through a separate pathway will facilitate investigations into the mechanisms through which G(12) proteins mediate diverse biological responses."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m500445200"xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "Meigs T.E."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "Casey P.J."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "Kaplan D.D."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "Juneja J."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "Stemmle L.N."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/author | "DeMarco C.T."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/pages | "18049-18055"xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/title | "Selective uncoupling of G alpha 12 from Rho-mediated signaling."xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15746095 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15746095 |
| http://purl.uniprot.org/citations/15746095 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15746095 |
| http://purl.uniprot.org/uniprot/#_E9PC54-mappedCitation-15746095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15746095 |
| http://purl.uniprot.org/uniprot/#_Q03113-mappedCitation-15746095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15746095 |
| http://purl.uniprot.org/uniprot/#_Q6ZQV4-mappedCitation-15746095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15746095 |
| http://purl.uniprot.org/uniprot/#_Q7LDZ6-mappedCitation-15746095 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15746095 |
| http://purl.uniprot.org/uniprot/Q7LDZ6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15746095 |
| http://purl.uniprot.org/uniprot/Q03113 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15746095 |
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| http://purl.uniprot.org/uniprot/Q6ZQV4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15746095 |