| http://purl.uniprot.org/citations/15754088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15754088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15754088 | http://www.w3.org/2000/01/rdf-schema#comment | "Sorcin is a 21.6-kDa Ca(2+) binding protein of the penta-EF hand family. Several studies have shown that sorcin modulates multiple proteins involved in excitation-contraction (E-C) coupling in the heart, such as the cardiac ryanodine receptor (RyR2), L-type Ca(2+) channel, and Na(+)-Ca(2+) exchanger, while it has also been shown to be phosphorylated by cAMP-dependent protein kinase (PKA). To elucidate the effects of sorcin and its PKA-dependent regulation on E-C coupling in the heart, we identified the PKA-phosphorylation site of sorcin, and found that serine178 was preferentially phosphorylated by PKA and dephosphorylated by protein phosphatase-1. Isoproterenol allowed sorcin to translocate to the sarcoplasmic reticulum (SR). In addition, adenovirus-mediated overexpression of sorcin in adult rat cardiomyocytes significantly increased both the rate of decay of the Ca(2+) transient and the SR Ca(2+) load. An assay of oxalate-facilitated Ca(2+) uptake showed that recombinant sorcin increased Ca(2+) uptake in a dose-dependent manner. These data suggest that sorcin activates the Ca(2+)-uptake function in the SR. In UM-X7. 1 cardiomyopathic hamster hearts, the relative amount of sorcin was significantly increased in the SR fraction, whereas it was significantly decreased in whole-heart homogenates. In failing hearts, PKA-phosphorylated sorcin was markedly increased, as assessed using a back-phosphorylation assay with immunoprecipitated sorcin. Our results suggest that sorcin activates Ca(2+)-ATPase-mediated Ca(2+) uptake and restores SR Ca(2+) content, and may play critical roles in compensatory mechanisms in both Ca(2+) homeostasis and cardiac dysfunction in failing hearts."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00395-005-0518-7"xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00395-005-0518-7"xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Matsuzaki M."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Matsuzaki M."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Matsumoto T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Matsumoto T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Inoue N."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Inoue N."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Suzuki S."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Suzuki S."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Ikeda Y."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Ikeda Y."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Hisamatsu Y."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Hisamatsu Y."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Ohkusa T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/author | "Ohkusa T."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/name | "Basic Res. Cardiol."xsd:string |
| http://purl.uniprot.org/citations/15754088 | http://purl.uniprot.org/core/name | "Basic Res. Cardiol."xsd:string |