| http://purl.uniprot.org/citations/1575483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1575483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1575483 | http://www.w3.org/2000/01/rdf-schema#comment | "The endoglucanase isolated from culture filtrates of Streptomyces lividans IAF74 was shown to have an Mr of 46,000 and a pI of 3.3. The specific enzyme activity of 539 IU/mg, determined by the reducing assay method on carboxymethyl cellulose, is among the highest reported in the literature. The cellulase showed typical endo-type activity when reacting on oligocellodextrins. Optimal enzyme activity was obtained at 50 degrees C and pH 5.5. The kinetic constants for this endoglucanase, determined with carboxymethyl cellulose as the substrate, were a Vmax of 24.9 IU/mg of enzyme and a Km of 4.2 mg/ml. Activity was found against neither methylumbelliferyl-nor p-nitrophenyl-cellobiopyranoside nor with xylan. The DNA sequence contains one possible reading frame validated by the N terminus of the mature purified protein. However, neither ATG nor GTG starting codons were identified near the ribosome-binding site. A putative TTG codon was found as a good candidate for the start codon. Comparison of the primary amino acid sequence of the endoglucanase of S. lividans revealed that the N terminus contains a bacterial cellulose-binding domain. The catalytic domain at the C terminus showed similarity to endoglucanases from a Bacillus sp. Thus, the endoglucanase CelA belongs to family A of cellulases as described before (N. R. Gilkes, B. Henrissat, D. G. Kilburn, R. C. Miller, Jr., and R. A. J. Warren, Microbiol. Rev. 55:303-315, 1991."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.58.3.815-820.1992"xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.58.3.815-820.1992"xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Shareck F."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Shareck F."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Kluepfel D."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Kluepfel D."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Morosoli R."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Morosoli R."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Lacaze P."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Lacaze P."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Theberge M."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/author | "Theberge M."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/pages | "815-820"xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/pages | "815-820"xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/title | "Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/title | "Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene."xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/1575483 | http://purl.uniprot.org/core/volume | "58"xsd:string |