| http://purl.uniprot.org/citations/15763467 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15763467 | http://www.w3.org/2000/01/rdf-schema#comment | "This paper reports the role of the tyramine (TA) receptor cascade in the insecticidal activity of plant essential oils. A TA receptor cDNA encoding a putative seven transmembrane domain G-protein coupled receptor was amplified from Drosophila melanogaster head cDNA phage library. The encoded protein contains 601 amino acids and has a sequence similar to other biogenic amine receptors. This protein was expressed in Drosophila S2 cells for radioligand binding studies with the ligand 3H-TA. Competitive binding studies comparing biogenic amines that could potentially function as endogenous ligands have demonstrated that this receptor had the highest affinity for TA (Ki=1.27 microM) followed by DL-octopamine, dopamine, serotonin and histamine. TA decreased the forskolin-increased cAMP levels (IC50=5.802 microM) and increased [Ca2+]i through the receptor expressed in S2 cells. The toxicity rank order of the tested plant essential oils against wild type D. melanogaster fly demonstrated a pattern similar to their effect on receptor binding activity and changes in cAMP level and [Ca2+]i. The toxicity of two of these chemicals was eliminated when tested against the TA receptor mutant (TyrRneo30) Drosophila strain. Therefore, the data indicates a correlation between cellular changes and insecticidal activity of tested plant essential oils, and suggests that the toxicity of at least two of these chemicals is mediated through the TA receptor."xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ibmb.2004.12.007"xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/author | "Enan E.E."xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/name | "Insect Biochem Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/pages | "309-321"xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/title | "Molecular response of Drosophila melanogaster tyramine receptor cascade to plant essential oils."xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://purl.uniprot.org/core/volume | "35"xsd:string |
| http://purl.uniprot.org/citations/15763467 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15763467 |
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