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http://purl.uniprot.org/citations/15774904http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15774904http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Liver X receptor (LXR) regulates the transcription of ATP-binding cassette transporter A1 (ABCA1) by binding to the DR-4 promoter element as a heterodimer with retinoid X receptor (RXR). The role of chromatin remodeling complex in LXR or ABCA1 activation has not been established previously. In this study, we investigated the activation of ABCA1 by brahma-related gene 1 (BRG-1) and brahma, members of the SWI/SNF (mating type switching/sucrose nonfermenting) chromatin remodeling complex.

Methods and results

Overexpression of wild-type BRG-1 in SW-13 cells, but not a catalytically inactive mutant, increased ABCA1 mRNA levels determined by RT-PCR. These effects were enhanced by LXR and RXR agonists. In 293T (epithelial kidney cell line) and Hep3B (hepatocyte cell line) cells, small interfering RNA against BRG-1/brm also affected ABCA1 mRNA levels. Synergistic activation of ABCA1 was obtained after coexpressing BRG-1 and SRC-1, a coactivator of LXR. Luciferase assays showed that this activation of ABCA1 was dependent on the promoter DR-4 element. Coimmunoprecipitation and chromatin immunoprecipitation studies indicated that the mechanism of BRG-1-mediated activation of ABCA1 involved interaction of LXR/RXR with BRG-1 and binding of this complex to ABCA1 promoter.

Conclusions

Catalytic subunits of SWI/SNF chromatin remodeling complex, BRG-1 and brahma, play significant roles in enhancing LXR/RXR-mediated transcription of ABCA1 via the promoter DR-4 element."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.org/dc/terms/identifier"doi:10.1161/01.atv.0000163186.58462.c5"xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/author"Fielding C.J."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/author"Fielding P.E."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/author"Huuskonen J."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/author"Vishnu M."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/name"Arterioscler Thromb Vasc Biol"xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/pages"1180-1185"xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/title"Activation of ATP-binding cassette transporter A1 transcription by chromatin remodeling complex."xsd:string
http://purl.uniprot.org/citations/15774904http://purl.uniprot.org/core/volume"25"xsd:string
http://purl.uniprot.org/citations/15774904http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15774904
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