| http://purl.uniprot.org/citations/15782178 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15782178 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15782178 | http://www.w3.org/2000/01/rdf-schema#comment | "Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods. To illustrate this platform, we describe its application to a specific target (At3g01050.1) from Arabidopsis thaliana. After cloning the target gene into a specialized plasmid, we carry out a small-scale (50 mul) in vitro sequential transcription and translation trial to ascertain the level of protein production and solubility. Next, we prepare mRNA for use in a 4-ml semicontinuous cell-free translation reaction to incorporate (15)N-labeled amino acids into a protein sample that we purify and test for suitability for NMR structural analysis. We then repeat the cell-free approach with (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample. The three-dimensional (3D) structure of At3g01050.1 shows that this protein is an unusual member of the beta-grasp protein family."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.org/dc/terms/identifier | "doi:10.1038/nmeth716"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.org/dc/terms/identifier | "doi:10.1038/nmeth716"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Markley J.L."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Markley J.L."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Lytle B.L."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Lytle B.L."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Peterson F.C."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Peterson F.C."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Tyler E.M."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Tyler E.M."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Vinarov D.A."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Vinarov D.A."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Volkman B.F."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/author | "Volkman B.F."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/name | "Nat. Methods"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/name | "Nat. Methods"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/pages | "149-153"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/pages | "149-153"xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/title | "Cell-free protein production and labeling protocol for NMR-based structural proteomics."xsd:string |
| http://purl.uniprot.org/citations/15782178 | http://purl.uniprot.org/core/title | "Cell-free protein production and labeling protocol for NMR-based structural proteomics."xsd:string |