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http://purl.uniprot.org/citations/15795295http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15795295http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15795295http://www.w3.org/2000/01/rdf-schema#comment"Wild-type equine herpesvirus 1 (EHV-1) strains express a large (250-kDa) glycoprotein, gp2, that is encoded by EUs4 (gene 71) located within the unique short region of the genome. DNA sequence analysis revealed that EUs4 of the pathogenic EHV-1 strain RacL11 is an open reading frame of 2,376 bp that encodes a protein of 791 amino acids. The attenuated EHV-1 vaccine strain KyA harbors an in-frame deletion of 1,242 bp from bp 222 to 1461 and expresses a truncated gp2 of 383 amino acids. To determine the relative contribution of gp2 to EHV-1 pathogenesis, we compared the course of respiratory infection of CBA mice infected with either wild-type RacL11, attenuated KyA, or a recombinant KyA that expresses the full-length gp2 protein (KyARgp2F). Mice infected with KyA lost a negligible amount of body weight (0.18% total weight loss) on day 1 postinfection and regained weight thereafter, whereas mice infected with KyARgp2F or RacL11 steadily lost weight beginning on day 1 and experienced a 20 and 18% loss in body weight, respectively, by day 3. Immunohistochemical and flow cytometric analyses revealed higher numbers of T and B lymphocytes and an extensive consolidation consisting of large numbers of Mac-1-positive cells in the lungs of animals infected with KyARgp2F compared to animals infected with KyA. RNase protection analyses revealed increased expression of numerous cytokines and chemokines, including interleukin-1beta (IL-1beta), IL-6, tumor necrosis factor alpha, macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, MIP-2, interferon gamma-inducible protein, monocyte chemotactic protein 1, and T-cell activation gene 3 at 12 h postinfection with KyARgp2F. Three independent DNA array experiments confirmed these results and showed a 2-to 13-fold increase in the expression of 31 inflammatory genes at 8 and 12 h postinfection with KyARgp2F compared to infection with KyA. Taken together, the results indicate that expression of full-length gp2 is sufficient to restore full respiratory virulence to the attenuated KyA strain and raise caution concerning the inclusion of full-length gp2 in the development of EHV-1 vaccines."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.org/dc/terms/identifier"doi:10.1128/jvi.79.8.5105-5115.2005"xsd:string
http://purl.uniprot.org/citations/15795295http://purl.org/dc/terms/identifier"doi:10.1128/jvi.79.8.5105-5115.2005"xsd:string
http://purl.uniprot.org/citations/15795295http://purl.org/dc/terms/identifier"doi:10.1128/JVI.79.8.5105-5115.2005"xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Smith P.M."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Smith P.M."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"O'Callaghan D.J."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"O'Callaghan D.J."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Osterrieder N."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Osterrieder N."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"von Einem J."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"von Einem J."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Kahan S.M."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Kahan S.M."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Rorex C.B."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/author"Rorex C.B."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/name"J. Virol."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/name"J. Virol."xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/pages"5105-5115"xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/pages"5105-5115"xsd:string
http://purl.uniprot.org/citations/15795295http://purl.uniprot.org/core/title"Expression of the full-length form of gp2 of equine herpesvirus 1 (EHV-1) completely restores respiratory virulence to the attenuated EHV-1 strain KyA in CBA mice."xsd:string
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