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http://purl.uniprot.org/citations/15797017http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15797017http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15797017http://www.w3.org/2000/01/rdf-schema#comment"

Background

Wnt/beta-catenin signaling regulates many processes during vertebrate development, including patterning of the mesoderm along the dorso-ventral axis and patterning of the neuroectoderm along the anterior-posterior axis during gastrulation. However, relatively little is known about Wnt target genes mediating these effects.

Results

Using zebrafish DNA microarrays, we have identified several new targets of Wnt/beta-catenin signaling, including sp5-like (sp5l, previously called spr2), a zinc-finger transcription factor of the Sp1 family. sp5-like is a direct target of Wnt/beta-catenin signaling and acts together with its paralog sp5 (previously called bts1) downstream of wnt8 in patterning of the mesoderm and neuroectoderm because (1) overexpression of sp5-like, like overexpression of wnt8, posteriorizes the neuroectoderm, (2) sp5-like morpholino-mediated knockdown, like wnt8 knockdown, causes anteriorization of the hindbrain, (3) combined knockdown of sp5 and sp5-like, like loss of wnt8, causes expansion of dorsal mesoderm, (4) sp5-like knockdown reduces the defects in mesoderm and neuroectoderm patterning caused by wnt8 overexpression, and (5) inhibition of sp5-like enhances the effects of hypomorphic loss of wnt8. Importantly, (6) overexpression of sp5-like is able to partially restore normal hindbrain patterning in wnt8 morphants.

Conclusions

sp5-like is a direct target of Wnt/beta-catenin signaling during gastrulation and, together with sp5, acts as a required mediator of the activities of wnt8 in patterning the mesoderm and neuroectoderm. We conclude that sp5 transcription factors mediate the downstream responses to Wnt/beta-catenin signaling in several developmental processes in zebrafish."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2005.01.041"xsd:string
http://purl.uniprot.org/citations/15797017http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2005.01.041"xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Weidinger G."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Weidinger G."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Moon R.T."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Moon R.T."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Thorpe C.J."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Thorpe C.J."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Wuennenberg-Stapleton K."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Wuennenberg-Stapleton K."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Ngai J."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/author"Ngai J."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/pages"489-500"xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/pages"489-500"xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/title"The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/title"The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning."xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/15797017http://purl.uniprot.org/core/volume"15"xsd:string
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