| http://purl.uniprot.org/citations/15800629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15800629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15800629 | http://www.w3.org/2000/01/rdf-schema#comment | "RNA interference (RNAi) is a conserved sequence-specific gene regulatory mechanism mediated by the RNA-induced silencing complex (RISC), which is composed of a single-stranded guide RNA and an Argonaute protein. The PIWI domain, a highly conserved motif within Argonaute, has been shown to adopt an RNase H fold critical for the endonuclease cleavage activity of RISC. Here we report the crystal structure of Archaeoglobus fulgidus Piwi protein bound to double-stranded RNA, thereby identifying the binding pocket for guide-strand 5'-end recognition and providing insight into guide-strand-mediated messenger RNA target recognition. The phosphorylated 5' end of the guide RNA is anchored within a highly conserved basic pocket, supplemented by the carboxy-terminal carboxylate and a bound divalent cation. The first nucleotide from the 5' end of the guide RNA is unpaired and stacks over a conserved tyrosine residue, whereas successive nucleotides form a four-base-pair RNA duplex. Mutation of the corresponding amino acids that contact the 5' phosphate in human Ago2 resulted in attenuated mRNA cleavage activity. Our structure of the Piwi-RNA complex, and that determined elsewhere, provide direct support for the 5' region of the guide RNA serving as a nucleation site for pairing with target mRNA and for a fixed distance separating the RISC-mediated mRNA cleavage site from the anchored 5' end of the guide RNA."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature03514"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature03514"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Patel D.J."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Patel D.J."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Pei Y."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Pei Y."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Tuschl T."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Tuschl T."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Meister G."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Meister G."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Yuan Y.-R."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Yuan Y.-R."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Ma J.-B."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/author | "Ma J.-B."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/pages | "666-670"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/pages | "666-670"xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/title | "Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein."xsd:string |
| http://purl.uniprot.org/citations/15800629 | http://purl.uniprot.org/core/title | "Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein."xsd:string |