| http://purl.uniprot.org/citations/15809060 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15809060 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15809060 | http://www.w3.org/2000/01/rdf-schema#comment | "Homo-oligomerization via a coiled-coil (C-C) domain has been shown to be necessary for the promyelocytic leukemia (PML)-retinoic acid receptor-alpha (RARalpha) fusion protein to acquire oncogenic potential in acute promyelocytic leukemia. We show here that PML(DeltaC-C)-RARalpha, which contains a deletion in its C-C domain, is neither localized as characteristic microspeckles nor modified by small ubiquitin-like modifiers (SUMO). The absence of sumoylation of the DeltaC-C mutant was due to the lack of binding to Ubc9, a SUMO conjugation enzyme. The integrity of RING finger domain was also needed for both sumoylation and microspeckle formation. In GAL4-DNA tethering assays, the DeltaC-C mutant completely lost the inhibitory effect on retinoic acid (RA)-mediated transactivation. Furthermore, the expression of CD14 in U937 cells expressing the DeltaC-C mutant in response to vitamin D3 was markedly higher than in cells expressing PML-RARalpha. However, the RA-mediated induction of C/EBPbeta in cells expressing the DeltaC-C mutant was comparable to that of control cells. Thus, our results suggest that the C-C domain-associated functions of sumoylation, localization as microspeckles, and the inhibition of monocyte differentiation all contribute to the oncogenic activity of PML-RARalpha."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2005.03.052"xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2005.03.052"xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Lee J.M."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Lee J.M."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim D.Y."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim D.Y."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Han T.H."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Han T.H."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Ahn J.H."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Ahn J.H."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim S.T."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim S.T."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim Y.E."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/author | "Kim Y.E."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/pages | "746-754"xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/pages | "746-754"xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/title | "Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation."xsd:string |
| http://purl.uniprot.org/citations/15809060 | http://purl.uniprot.org/core/title | "Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation."xsd:string |