| http://purl.uniprot.org/citations/15826950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15826950 | http://www.w3.org/2000/01/rdf-schema#comment | "Glucocorticoids are the mainstay of asthma therapy and mediate the repression of a number of cytokine genes, such as Interleukin (IL)-4, -5, -13, and granulocyte macrophage colony-stimulating factor (GM-CSF), which are central to the pathogenesis of asthmatic airway inflammation. The glucocorticoid receptor (GR) mediates repression by a number of diverse mechanisms. We have previously suggested that one such repressive activity is by direct binding of GR to elements within the GM-CSF enhancer that are recognized by the nuclear factor of activated T cells.activator protein 1 (NF-AT.AP-1) complex. We reasoned that, because many cytokine genes activated in asthma are transcriptionally regulated by the recruitment of this complex to DNA, their binding sites might provide a target for GR to mediate its repressive effects. Here, we show that transcriptional repression of the Interleukin-5 gene involves recruitment of GR to a DNA region located within the IL-5 proximal promoter, which is bound by NF-AT and AP-1 proteins. GR recruitment had a profound effect upon the activation capacity of GATA3, which has a binding site close to the NF-AT.AP-1 domain in both IL-5 and IL-13 promoters. Repression by GR involves co-repressor recruitment, because treatment of transfected cells with the deacetylase inhibitor trichostatin A caused a partial relief of repression. Additionally, repression could be augmented by co-transfection of cells with a histone deacetylase (HDAC1). These data suggest that the local recruitment of GR causes repression by inhibiting transcriptional activation by GATA3, a key tissue-specific determinant of expression of Th2 cytokines."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m503659200"xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Bowen H."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Smith P."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Kelly A."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Lee T."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Richards D."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Soh C."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Lavender P."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Gilmour J."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Cousins D."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Hawrylowicz C."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/author | "Jee Y.K."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/pages | "23243-23250"xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/title | "Repression of interleukin-5 transcription by the glucocorticoid receptor targets GATA3 signaling and involves histone deacetylase recruitment."xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15826950 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15826950 |
| http://purl.uniprot.org/citations/15826950 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15826950 |
| http://purl.uniprot.org/uniprot/#_A2NCZ2-mappedCitation-15826950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15826950 |
| http://purl.uniprot.org/uniprot/#_A0A411AGV5-mappedCitation-15826950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15826950 |
| http://purl.uniprot.org/uniprot/#_A0A411AGV9-mappedCitation-15826950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15826950 |
| http://purl.uniprot.org/uniprot/#_A0A411AGW6-mappedCitation-15826950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15826950 |