| http://purl.uniprot.org/citations/15840581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15840581 | http://www.w3.org/2000/01/rdf-schema#comment | "The high molecular mass glycosaminoglycan hyaluronan (HA) can become modified by the covalent attachment of heavy chains (HCs) derived from the serum protein inter-alpha-inhibitor (IalphaI), which is composed of three subunits (HC1, HC2 and bikunin) linked together via a chondroitin sulfate moiety. The formation of HC.HA is likely to play an important role in the stabilization of HA-rich extracellular matrices in the context of inflammatory disease (e.g. arthritis) and ovulation. Here, we have characterized the complexes formed in vitro between purified human IalphaI and recombinant human TSG-6 (an inflammation-associated protein implicated previously in this process) and show that these complexes (i.e. TSG-6 x HC1 and TSG-6 x HC2) act as intermediates in the formation of HC x HA. This is likely to involve two transesterification reactions in which an ester bond linking an HC to chondroitin sulfate in intact IalphaI is transferred first onto TSG-6 and then onto HA. The formation of TSG-6 x HC1 and TSG-6 x C2 complexes was accompanied by the production of bikunin x HC2 and bikunin x HC1 by-products, respectively, which were observed to break down, releasing free bikunin and HCs. Both TSG-6 x HC formation and the subsequent HC transfer are metal ion-dependent processes; these reactions have a requirement for either Mg2+ or Mn2+ and are inhibited by Co2+. TSG-6, which is released upon the transfer of HCs from TSG-6 onto HA, was shown to combine with IalphaI to form new TSG-6 x HC complexes and thus be recycled. The finding that TSG-6 acts as cofactor and catalyst in the production of HC x HA complexes has important implications for our understanding of inflammatory and inflammation-like processes."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m501332200"xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Mukhopadhyay D."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Willis A.C."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Fries E."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Day A.J."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Milner C.M."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Rugg M.S."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Hascall V.C."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/author | "Fulop C."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/pages | "25674-25686"xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/title | "Characterization of complexes formed between TSG-6 and inter-alpha-inhibitor that act as intermediates in the covalent transfer of heavy chains onto hyaluronan."xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15840581 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15840581 |
| http://purl.uniprot.org/citations/15840581 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15840581 |
| http://purl.uniprot.org/uniprot/#_A2RTY6-mappedCitation-15840581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/#_B4DM79-mappedCitation-15840581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/#_P98066-mappedCitation-15840581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/#_P19823-mappedCitation-15840581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/P98066 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/A2RTY6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15840581 |
| http://purl.uniprot.org/uniprot/P19823 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15840581 |