| http://purl.uniprot.org/citations/15843375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15843375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15843375 | http://www.w3.org/2000/01/rdf-schema#comment | "In all organisms studied, elevated temperatures induce the expression of a variety of stress proteins, among them small Hsps (sHsp). sHsps are chaperones that prevent the unspecific aggregation of proteins by forming stable complexes with unfolded polypeptides. Reactivation of captured proteins requires the assistance of other ATP-dependent chaperones. How sHsps and ATP-dependent chaperones work together is poorly understood. Here, we analyzed the interplay of chaperones present in the cytosol of Saccharomyces cerevisiae. Specifically, we characterized the influence of Hsp104 and Ssa1 on the disassembly of Hsp26 x substrate complexes in vitro and in vivo. We show that recovery of proteins from aggregates in the cell requires the chaperones to work together with defined but overlapping functions. During reactivation, proteins are transferred from a stable complex with Hsp26 to Hsp104 and Hsp70. The need for ATP-dependent chaperones depends on the type of sHsp x substrate complex. Although Ssa1 is able to release substrate proteins from soluble Hsp26 x substrate complexes, Hsp104 is essential to dissociate substrate proteins from aggregates with incorporated sHsps. Our results are consistent with a model of several interrelated defense lines against protein aggregation."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m502697200"xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m502697200"xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Haslbeck M."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Haslbeck M."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Walter S."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Walter S."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Buchner J."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Buchner J."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Miess A."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Miess A."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Stromer T."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/author | "Stromer T."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/pages | "23861-23868"xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/pages | "23861-23868"xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/title | "Disassembling protein aggregates in the yeast cytosol. The cooperation of Hsp26 with Ssa1 and Hsp104."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/title | "Disassembling protein aggregates in the yeast cytosol. The cooperation of Hsp26 with Ssa1 and Hsp104."xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15843375 | http://purl.uniprot.org/core/volume | "280"xsd:string |