| http://purl.uniprot.org/citations/15848748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15848748 | http://www.w3.org/2000/01/rdf-schema#comment | "A major catabolic enzyme of the plant hormone abscisic acid (ABA) is the cytochrome P450 monooxygenase ABA 8'-hydroxylase. For designing a specific inhibitor of this enzyme, the substrate specificity and inhibition of CYP707A3, an ABA 8'-hydroxylase from Arabidopsis thaliana, was investigated using 45 structural analogues of ABA and compared to the structural requirements for ABA activity. Substrate recognition by the enzyme strictly required the 6'-methyl groups (C-8' and C-9'), which were unnecessary for ABA activity, whereas elimination of the 3-methyl (C-6) and 1'-hydroxyl groups, which significantly affected ABA activity, had little effect on the ability of analogues to competitively inhibit the enzyme. Fluorination at C-8' and C-9' resulted in resistance to 8'-hydroxylation and competitive inhibition of the enzyme. In particular, 8',8'-difluoro-ABA and 9',9'-difluoro-ABA yielded no enzyme reaction products and strongly inhibited the enzyme (K(I) = 0.16 and 0.25 microM, respectively)."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bmc.2005.03.015"xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Hirai N."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Sakata K."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Saito S."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Araki Y."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Mizutani M."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Ueno K."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/author | "Todoroki Y."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/name | "Bioorg Med Chem"xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/pages | "3359-3370"xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/title | "Differences between the structural requirements for ABA 8'-hydroxylase inhibition and for ABA activity."xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/15848748 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15848748 |
| http://purl.uniprot.org/citations/15848748 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15848748 |
| http://purl.uniprot.org/uniprot/#_Q9FH76-mappedCitation-15848748 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15848748 |
| http://purl.uniprot.org/uniprot/Q9FH76 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15848748 |