| http://purl.uniprot.org/citations/15849195 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15849195 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15849195 | http://www.w3.org/2000/01/rdf-schema#comment | "ADAP (adhesion and degranulation-promoting adaptor protein) and SKAP55 (Src kinase-associated phosphoprotein of 55 kDa) are T cell adaptors that mediate inside-out signaling from the T cell antigen receptor to integrins, giving rise to increased integrin affinity/avidity and formation of the immunological synapse between the T cell and the antigen-presenting cell. These two proteins are tightly and constitutively associated with one another, and their ability to interact is required for inside-out signaling. Here we show in an ADAP-deficient Jurkat T cell line that the co-dependence of ADAP and SKAP55 extends beyond their functional and physical interactions and show that SKAP55 protein is unstable in the absence of ADAP. Restoration of ADAP to the ADAP-deficient Jurkat T cell line restores SKAP55 expression by causing a 5-fold decrease in the rate of SKAP55 proteolysis. Inactivation of the Src homology 3 domain of SKAP55, which mediates the association between SKAP55 with ADAP, blocks the protective effect of ADAP. The half-life of SKAP55, in the absence of ADAP, is approximately 15-20 min, increasing to 90 min in the presence of ADAP. This is a remarkably rapid rate of turnover for a signaling protein and suggests the possibility that stimuli that signal for the stabilization of SKAP55 may play an important role in T cell adhesion and conjugate formation."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m413201200"xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m413201200"xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Wange R.L."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Wange R.L."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Martindale J.L."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Martindale J.L."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Precht P."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Precht P."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Burkhardt J.K."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Burkhardt J.K."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Norton D.D."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/author | "Norton D.D."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/pages | "23576-23583"xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/pages | "23576-23583"xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/title | "Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells."xsd:string |
| http://purl.uniprot.org/citations/15849195 | http://purl.uniprot.org/core/title | "Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells."xsd:string |