Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/15855509http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15855509http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15855509http://www.w3.org/2000/01/rdf-schema#comment"Seventeen extended-spectrum beta-lactamase (ESBL)-producing isolates of the family Enterobacteriaceae recovered from 1998 to 2000 in hospitals of five different cities in Poland were analyzed. They expressed several TEM-type ESBLs, TEM-4, TEM-29, TEM-85, TEM-86, TEM-93, and TEM-94. TEM-85 (L21F, R164S, E240K, T265M), TEM-86 (L21F, R164S, A237T, E240K, T265M), TEM-93 (M182T, G238S, E240K), and TEM-94 (L21F, E104K, M182T, G238S, T265M) were identified for the first time. Including the enzymes described earlier, TEM-47, TEM-48, TEM-49, and TEM-68, the group of known ESBLs of the TEM family produced by enterobacteria in Polish hospitals has increased to 10 variants. Comparative sequence analysis of the genes coding for all these beta-lactamases revealed a view of their possible evolution, which, apart from the gradual acquisition of various mutations, could also have involved recombination events. Two different bla(TEM-1) gene alleles were precursors of the ESBL genes: bla(TEM-1A), which was the ancestor of bla(TEM-93), and bla(TEM-1F), from which all the remaining genes originated. The evolution of the bla(TEM-1F)-related genes most probably consisted of three major separate lineages, one of which, including bla(TEM-4), bla(TEM-47), bla(TEM-48), bla(TEM-49), bla(TEM-68), and bla(TEM-94), was highly structured itself and could have been initiated by the bla(TEM-25) gene, identified exclusively in France so far. Plasmid fingerprinting analysis revealed a high degree of diversity of plasmids carrying related bla(TEM) genes, which suggested either the intense diversification or transposition of bla(TEM) genes between different plasmids or some contribution of convergent evolution. The results of this study clearly demonstrate that the environment of Polish hospitals has been highly favorable for the rapid evolution of ESBLs."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.org/dc/terms/identifier"doi:10.1128/AAC.49.5.1872-1880.2005"xsd:string
http://purl.uniprot.org/citations/15855509http://purl.org/dc/terms/identifier"doi:10.1128/aac.49.5.1872-1880.2005"xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Hryniewicz W."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Hryniewicz W."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Gniadkowski M."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Gniadkowski M."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Baraniak A."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Baraniak A."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Fiett J."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Fiett J."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Mrowka A."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Mrowka A."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Walory J."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/author"Walory J."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/name"Antimicrob. Agents Chemother."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/name"Antimicrob Agents Chemother"xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/pages"1872-1880"xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/pages"1872-1880"xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/title"Evolution of TEM-type extended-spectrum beta-lactamases in clinical Enterobacteriaceae strains in Poland."xsd:string
http://purl.uniprot.org/citations/15855509http://purl.uniprot.org/core/title"Evolution of TEM-type extended-spectrum beta-lactamases in clinical Enterobacteriaceae strains in Poland."xsd:string
▶