| http://purl.uniprot.org/citations/15859335 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15859335 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15859335 | http://www.w3.org/2000/01/rdf-schema#comment | "Hainantoxin-IV (HNTX-IV) purified from the venom of the spider Selenocosmia hainana is a potent antagonist that acts on tetrodotoxin-sensitive (TrX-S) sodium channels. It is a 35-residue polypeptide and includes three disulfide bridges. In order to investigate the structure-function relationship of HNTX-IV, two mutants (S12A-HNTX-IV and R29A-HNTX-IV) of HNTX-TV in which Ser12 and Arg29 were replaced by Ala respectively, were synthesized by solid-phase Fmoc chemistry, followed by oxidative refolding of purified peptides under the optimal conditions. The synthetic mutants were analyzed by MALDI-TOF mass spectrometry, nuclear magnetic resonance spectroscopy (NMR) and electrophysiological experiments for molecular weight, conformation and physiological activity, respectively. The results show that the mutants and native HNTX-IV (nHNTX-IV) have almost identical three-dimensional structures. The bioactivity level of S12A-HNTX-IV is also about the same as that of nHNTX-IV, suggesting that Ser12 does not play any important role for the bioactivity of this toxin. The bioactivity of R29A-HNTX-IV is reduced by at last 155 times, indicating that Arg29 is a key residue relative to the bioactivity of HNTX-IV. It is presumed that the decrease in activity of R29A-HNTX-IV is due to the changes of the property in the binding site rather than the change in the basic conformation of the molecule."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xiong X."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xiong X."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xu X."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Li D.L."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Li D.L."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Wang X.C."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Wang X.C."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Liang S.P."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Liang S.P."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xiao Y.C."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/author | "Xiao Y.C."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/name | "Sheng Wu Gong Cheng Xue Bao"xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/name | "Sheng Wu Gong Cheng Xue Bao"xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/pages | "92-96"xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/pages | "92-96"xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/title | "Solid-phase synthesis and biological characterization of S12A-HNTX-IV and R29A-HNTX-IV: two mutants of hainantoxin-IV."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/title | "Solid-phase synthesis and biological characterization of S12A-HNTX-IV and R29A-HNTX-IV: two mutants of hainantoxin-IV."xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/15859335 | http://purl.uniprot.org/core/volume | "21"xsd:string |