| http://purl.uniprot.org/citations/15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15860731 | http://www.w3.org/2000/01/rdf-schema#comment | "The lumenal ionic content of an organelle is determined by its complement of channels and transporters. These proteins reach their resident organelles by adaptor-dependent mechanisms. This concept is illustrated in AP-3 deficiencies, in which synaptic vesicle zinc is depleted because the synaptic-vesicle-specific zinc transporter 3 does not reach synaptic vesicles. However, whether zinc transporter 3 is the only membrane protein defining synaptic-vesicle zinc content remains unknown. To address this question, we examined whether zinc transporter 3 and the vesicular glutamate transporter Vglut1 (a transporter that coexists with zinc transporter 3 in brain nerve terminals) were co-targeted to synaptic-like microvesicle fractions in PC12 cells. Deconvolution microscopy and subcellular fractionation demonstrated that these two transporters were present on the same vesicles in PC12 cells. Vglut1 content in synaptic-like microvesicle fractions and brain synaptic vesicles was partially sensitive to pharmacological and genetic perturbation of AP-3 function. Whole-cell flow-cytometry analysis of PC12 cell lines expressing zinc transporter 3, Vglut1 or both showed that vesicular zinc uptake was increased by Vglut1 expression. Conversely, production of zinc transporter 3 increased the vesicular uptake of glutamate in a zinc-dependent fashion. Our results suggest that the coupling of zinc transporter 3 and Vglut1 transport mechanisms regulates neurotransmitter content in secretory vesicles."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.02319"xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/author | "Love R."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/author | "Faundez V."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/author | "Salazar G."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/author | "Kalman D."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/author | "Craige B."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/pages | "1911-1921"xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/title | "Vglut1 and ZnT3 co-targeting mechanisms regulate vesicular zinc stores in PC12 cells."xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://purl.uniprot.org/core/volume | "118"xsd:string |
| http://purl.uniprot.org/citations/15860731 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15860731 |
| http://purl.uniprot.org/citations/15860731 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15860731 |
| http://purl.uniprot.org/uniprot/O54774#attribution-CCA320D9593FD1FF70A0093F61F6D33A | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/Q3TXX4#attribution-72B1A534E29BBA012836D50B6A650A6F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/P97441#attribution-53D3790F4E7046A83271BB3B1675E7C3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GRU0-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A571BEC1-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A571BES1-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A571BEW4-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P7Z2-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P6Q6-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |
| http://purl.uniprot.org/uniprot/#_A6JAY9-mappedCitation-15860731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15860731 |