| http://purl.uniprot.org/citations/15864302 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15864302 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15864302 | http://www.w3.org/2000/01/rdf-schema#comment | "Nuclear protein import is mediated mainly by the transport factor importin-beta that binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through nuclear pore complexes. This active transport is driven by disassembly of the import complex by nuclear RanGTP. The switch I and II loops of Ran change conformation with nucleotide state, and regulate its interactions with nuclear trafficking components. Importin-beta consists of 19 HEAT repeats that are based on a pair of antiparallel alpha-helices (referred to as the A- and B-helices). The HEAT repeats stack to yield two C-shaped arches, linked together to form a helicoidal molecule that has considerable conformational flexibility. Here we present the structure of full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides a basis for understanding the crucial cargo-release step of nuclear import. We identify a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p. This interaction produces a change in helicoidal pitch that locks Kap95p in a conformation that cannot bind importin-alpha or cargo. We suggest an allosteric mechanism for nuclear import complex disassembly by RanGTP."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature03578"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature03578"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Matsuura Y."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Matsuura Y."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Stewart M."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Stewart M."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Liu S.M."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/author | "Liu S.M."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/pages | "693-696"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/pages | "693-696"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/title | "Structural basis for nuclear import complex dissociation by RanGTP."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/title | "Structural basis for nuclear import complex dissociation by RanGTP."xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/volume | "435"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://purl.uniprot.org/core/volume | "435"xsd:string |
| http://purl.uniprot.org/citations/15864302 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15864302 |
| http://purl.uniprot.org/citations/15864302 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15864302 |