| http://purl.uniprot.org/citations/15866891 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15866891 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15866891 | http://www.w3.org/2000/01/rdf-schema#comment | "Store-operated Ca2+ (SOC) channels regulate many cellular processes, but the underlying molecular components are not well defined. Using an RNA interference (RNAi)-based screen to identify genes that alter thapsigargin (TG)-dependent Ca2+ entry, we discovered a required and conserved role of Stim in SOC influx. RNAi-mediated knockdown of Stim in Drosophila S2 cells significantly reduced TG-dependent Ca2+ entry. Patch-clamp recording revealed nearly complete suppression of the Drosophila Ca2+ release-activated Ca2+ (CRAC) current that has biophysical characteristics similar to CRAC current in human T cells. Similarly, knockdown of the human homologue STIM1 significantly reduced CRAC channel activity in Jurkat T cells. RNAi-mediated knockdown of STIM1 inhibited TG- or agonist-dependent Ca2+ entry in HEK293 or SH-SY5Y cells. Conversely, overexpression of STIM1 in HEK293 cells modestly enhanced TG-induced Ca2+ entry. We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.200502019"xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.200502019"xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Roos J."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Roos J."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Velicelebi G."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Velicelebi G."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Wagner S.L."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Wagner S.L."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Ohlsen K."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Ohlsen K."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Stauderman K.A."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Stauderman K.A."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Cahalan M.D."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Cahalan M.D."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Safrina O."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Safrina O."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Yeromin A.V."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "Yeromin A.V."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "DiGregorio P.J."xsd:string |
| http://purl.uniprot.org/citations/15866891 | http://purl.uniprot.org/core/author | "DiGregorio P.J."xsd:string |