| http://purl.uniprot.org/citations/15882992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15882992 | http://www.w3.org/2000/01/rdf-schema#comment | "CARD12 (Ipaf/Clan) is an important regulator of caspase-1 activation. It belongs to the family of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins. The NBS domain of the NBS-LRR proteins contains putative ATP/GTPase-specific P-loop and Mg2+-binding site motifs. However, the nucleotide-binding properties and the function of the NBS domain are unknown. We developed a nucleotide-binding assay and investigated nucleotide binding to CARD12. We find that the NBS domain of CARD12 contains a nucleotide-binding pocket with specificity for ATP/dATP. A point mutation in the P-loop (K175R) of the NBS domain abolishes ATP/dATP binding. We further demonstrate that the nucleotide-binding site is required for CARD12-mediated caspase-1 activation. CARD12 self-association and association with procaspase-1 in transfected cells were markedly decreased by the P-loop mutation K175R. Furthermore, the P-loop mutation greatly reduced caspase-1 activation-dependent proIL-1beta processing. Thus, CARD12 function is dependent on the nucleotide-binding site. Our data provide insights into the molecular mechanisms of CARD12-mediated caspase-1 activation."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2005.04.027"xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Lu C."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Wang A."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Ocain T.D."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/author | "Dorsch M."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/pages | "1114-1119"xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/title | "Nucleotide binding to CARD12 and its role in CARD12-mediated caspase-1 activation."xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://purl.uniprot.org/core/volume | "331"xsd:string |
| http://purl.uniprot.org/citations/15882992 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15882992 |
| http://purl.uniprot.org/citations/15882992 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15882992 |
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