| http://purl.uniprot.org/citations/15883163 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15883163 | http://www.w3.org/2000/01/rdf-schema#comment | "This study was aimed to characterize the mitochondrial and extra-mitochondrial oxygen consuming reactions in human CD34+ hematopoietic stem cells. Cell samples were collected by apheresis following pre-conditioning by granulocyte colony-stimulating factor and isolated by anti-CD34 positive immunoselection. Polarographic analysis of the CN-sensitive endogenous cell respiration revealed a low mitochondrial oxygen consumption rate. Differential absorbance spectrometry on whole cell lysate and two-dimensional blue native-PAGE analysis of mitoplast proteins confirmed a low amount of mitochondrial respiratory chain complexes thus qualifying the hematopoietic stem cell as a poor oxidative phosphorylating cell type. Confocal microscopy imaging showed, however, that the intracellular content of mitochondria was not homogeneously distributed in the CD34+ hematopoietic stem cell sample displaying a clear inverse correlation of their density with the expression of the CD34 commitment marker. About half of the endogenous oxygen consumption was extra-mitochondrial and completely inhibitable by enzymatic scavengers of reactive oxygen species and by diphenylene iodinium. By spectral analysis, flow cytometry, reverse transcriptase-PCR, immunocytochemistry, and immunoprecipitation it was shown that the extra-mitochondrial oxygen consumption was contributed by the NOX2 and NOX4 isoforms of the O2-*. producer plasma membrane NAD(P)H oxidase with low constitutive activity. A model is proposed suggesting for the NAD(P)H oxidase a role of O2 sensor and/or ROS source serving as redox messengers in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m500047200"xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Piccoli C."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Capitanio N."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Falzetti F."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Boffoli D."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Tabilio A."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Ria R."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Cela O."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "D'Aprile A."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/author | "Scrima R."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/pages | "26467-26476"xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/title | "Characterization of mitochondrial and extra-mitochondrial oxygen consuming reactions in human hematopoietic stem cells. Novel evidence of the occurrence of NAD(P)H oxidase activity."xsd:string |
| http://purl.uniprot.org/citations/15883163 | http://purl.uniprot.org/core/volume | "280"xsd:string |
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