| http://purl.uniprot.org/citations/15901763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15901763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15901763 | http://www.w3.org/2000/01/rdf-schema#comment | "Podocytes, the glomerular epithelial cells of the kidney, share important features with neuronal cells. In addition to phenotypical and functional similarities, a number of gene products have been found to be expressed exclusively or predominantly by both cell types. With the hypothesis of a common transcriptome shared by podocytes and neurons, digital differential display was used to identify novel podocyte-expressed gene products. Comparison of brain and kidney cDNA libraries with those of other organs identified Sam68-like mammalian protein 2 (SLM-2), a member of the STAR family of RNA processing proteins, as expressed by podocytes. SLM-2 expression was found to be restricted in the kidney to podocytes. In proteinuric diseases, SLM-2, a known regulator of neuronal mRNA splice site selection, was found significantly upregulated on mRNA and protein levels. Knockdown of SLM-2 by short interfering RNA in podocytes was performed to evaluate its biologic role. RNA splicing of vascular endothelial growth factor (VEGF), a key regulator of the filtration barrier and expressed as functionally distinct splice isoforms, was evaluated. VEGF(165) expression was found to be reduced by 25% after SLM-2 knockdown. In vivo, the glomerular expression of SLM-2 correlated with the mRNA levels of VEGF(165). This study demonstrates the power of digital differential display to predict cell type-specific gene expression by hypothesis-driven analysis of tissue cDNA libraries. SLM-2-dependent VEGF splicing indicates the importance of mRNA splice site selection for glomerular filtration barrier function."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.org/dc/terms/identifier | "doi:10.1681/asn.2005020204"xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.org/dc/terms/identifier | "doi:10.1681/asn.2005020204"xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Mathieson P.W."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Mathieson P.W."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Saleem M.A."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Saleem M.A."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Schmid H."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Schmid H."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Wang G.Q."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Wang G.Q."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Cohen C.D."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Cohen C.D."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Kretzler M."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Kretzler M."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Blattner S.M."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Blattner S.M."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Doran P.P."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Doran P.P."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Henger A."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Henger A."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Merkle M."xsd:string |
| http://purl.uniprot.org/citations/15901763 | http://purl.uniprot.org/core/author | "Merkle M."xsd:string |