| http://purl.uniprot.org/citations/15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15908920 | http://www.w3.org/2000/01/rdf-schema#comment | "A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT). To streamline this approach, we generated Cre-inducible DTR transgenic mice (iDTR) in which Cre-mediated excision of a STOP cassette renders cells sensitive to DT. We tested the iDTR strain by crossing to the T cell- and B cell-specific CD4-Cre and CD19-Cre strains, respectively, and observed efficient ablation of T and B cells after exposure to DT. In MOGi-Cre/iDTR double transgenic mice expressing Cre recombinase in oligodendrocytes, we observed myelin loss after intraperitoneal DT injections. Thus, DT crosses the blood-brain barrier and promotes cell ablation in the central nervous system. Notably, we show that the developing DT-specific antibody response is weak and not neutralizing, and thus does not impede the efficacy of DT. Our results validate the use of iDTR mice as a tool for cell ablation in vivo."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.org/dc/terms/identifier | "doi:10.1038/nmeth762"xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Jung S."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Buch T."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Heppner F.L."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Kremer M."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Waisman A."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Wunderlich F.T."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Tertilt C."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/author | "Heinen T.J."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/name | "Nat Methods"xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/pages | "419-426"xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/title | "A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration."xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://purl.uniprot.org/core/volume | "2"xsd:string |
| http://purl.uniprot.org/citations/15908920 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15908920 |
| http://purl.uniprot.org/citations/15908920 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15908920 |
| http://purl.uniprot.org/uniprot/#_D3YXP4-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_A0A0X1KG58-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_D3Z3N6-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_O88398-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_Q3U1A5-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_P25918-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |
| http://purl.uniprot.org/uniprot/#_Q3UY21-mappedCitation-15908920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15908920 |