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http://purl.uniprot.org/citations/15914861http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15914861http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15914861http://www.w3.org/2000/01/rdf-schema#comment"Transport of the viral genome into the nucleus is an obligatory step in the replication cycle of plant pararetro- and geminiviruses. In both these virus types, the multifunctional coat protein (CP) is thought to be involved in this process. Here, a green fluorescent protein tagging approach was used to demonstrate nuclear import of the CPs of Rice tungro bacilliform virus (RTBV) and Mungbean yellow mosaic virus--Vigna (MYMV) in Nicotiana plumbaginifolia protoplasts. In both cases, at least two nuclear localization signals (NLSs) were identified and characterized. The NLSs of RTBV CP are located within both N- and C-terminal regions (residues 479KRPK/497KRK and 744KRK/758RRK), and those of MYMV CP within the N-terminal part (residues 3KR and 41KRRR). The MYMV and RTBV CP NLSs resemble classic mono- and bipartite NLSs, respectively. However, the N-terminal MYMV CP NLS and both RTBV CP NLSs show peculiarities in the number and position of basic residues. In vitro pull-down assays revealed interaction of RTBV and MYMV CPs with the nuclear import factor importin alpha, suggesting that both CPs are imported into the nucleus via an importin alpha-dependent pathway. The possibility that this pathway could serve for docking of virions to the nucleus is discussed."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.org/dc/terms/identifier"doi:10.1099/vir.0.80920-0"xsd:string
http://purl.uniprot.org/citations/15914861http://purl.org/dc/terms/identifier"doi:10.1099/vir.0.80920-0"xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Guerra-Peraza O."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Guerra-Peraza O."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Herzog E."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Herzog E."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Hohn T."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Hohn T."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Kirk D."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Kirk D."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Schmit A.C."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Schmit A.C."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Seltzer V."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Seltzer V."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Veluthambi K."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/author"Veluthambi K."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/name"J. Gen. Virol."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/name"J. Gen. Virol."xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/pages"1815-1826"xsd:string
http://purl.uniprot.org/citations/15914861http://purl.uniprot.org/core/pages"1815-1826"xsd:string