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http://purl.uniprot.org/citations/15916946http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15916946http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15916946http://www.w3.org/2000/01/rdf-schema#comment"

Background

The Aurora kinases control multiple aspects of mitosis, among them centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. Aurora activity is regulated in part by a subset of Aurora substrates that, once phosphorylated, can enhance Aurora kinase activity. Aurora A substrate activators include TPX2 and Ajuba, whereas the only known Aurora B substrate activator is the chromosomal passenger INCENP.

Results

We report that the C. elegans Tousled kinase TLK-1 is a second substrate activator of the Aurora B kinase AIR-2. Tousled kinase (Tlk) expression and activity have been linked to ongoing DNA replication, and Tlk can phosphorylate the chromatin assembly factor Asf. Here, we show that TLK-1 is phosphorylated by AIR-2 during prophase/prometaphase and that phosphorylation increases TLK-1 kinase activity in vitro. Phosphorylated TLK-1 increases AIR-2 kinase activity in a manner that is independent of TLK-1 kinase activity but depends on the presence of ICP-1/INCENP. In vivo, TLK-1 and AIR-2 cooperate to ensure proper mitotic chromosome segregation.

Conclusions

The C. elegans Tousled kinase TLK-1 is a substrate and activator of the Aurora B kinase AIR-2. These results suggest that Tousled kinases have a previously unrecognized role in mitosis and that Aurora B associates with discrete regulatory complexes that may impart distinct substrate specificities and functions to the Aurora B kinase."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2005.04.019"xsd:string
http://purl.uniprot.org/citations/15916946http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2005.04.019"xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Han Z."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Han Z."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Schumacher J.M."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Schumacher J.M."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Mango S.E."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Mango S.E."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Riefler G.M."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Riefler G.M."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Saam J.R."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/author"Saam J.R."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/pages"894-904"xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/pages"894-904"xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/title"The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/title"The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase."xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/15916946http://purl.uniprot.org/core/volume"15"xsd:string
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