http://purl.uniprot.org/citations/15919996 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15919996 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15919996 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/15919996 | http://www.w3.org/2000/01/rdf-schema#comment | "The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.1109730"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.1109730"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Daley D.O."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Daley D.O."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Drew D."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Drew D."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Granseth E."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Granseth E."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Melen K."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Melen K."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Rapp M."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "Rapp M."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "von Heijne G."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/author | "von Heijne G."xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/name | "Science"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/name | "Science"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/pages | "1321-1323"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/pages | "1321-1323"xsd:string |
http://purl.uniprot.org/citations/15919996 | http://purl.uniprot.org/core/title | "Global topology analysis of the Escherichia coli inner membrane proteome."xsd:string |