| http://purl.uniprot.org/citations/15933156 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15933156 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15933156 | http://www.w3.org/2000/01/rdf-schema#comment | "Animal peptide toxins have become powerful tools to study structure-function relationships and physiological roles of voltage-activated Ca(2+) channels. In the present study, we investigated the effects of PnTx3-6, a neurotoxin purified from the venom of the spider Phoneutria nigriventer on cloned mammalian Ca(2+) channels expressed in human embryonic kidney 293 cells and endogenous Ca(2+) channels in N18 neuroblastoma cells. Whole-cell patch-clamp measurements indicate that PnTx3-6 reversibly inhibited L-(alpha(1C)/Ca(v)1.2), N-(alpha(1B)/Ca(v)2.2), P/Q-(alpha(1A)/Ca(v)2.1), and R-(alpha(1E)/Ca(v)2.3) type channels with varying potency (alpha(1B) > alpha(1E) > alpha(1A) > alpha(1C)) and IC(50) values of 122, 136, 263, and 607 nM, respectively. Inhibition occurred without alteration of the kinetics or the voltage dependence of the exogenously expressed Ca(2+) channels. In N18 cells, PnTx3-6 exhibited highest potency against N-type (conotoxin-GVIA-sensitive) current. In contrast to its effects on high voltage-activated Ca(2+) channels subtypes, application of 1 microM PnTx3-6 did not affect alpha(1G)/Ca(v)3.1 T-type Ca(2+) channels. Based on our study, we suggest that PnTx3-6 acts as a omega-toxin that targets high voltage-activated Ca(2+) channels, with a preference for the Ca(v)2 subfamily (N-, P/Q-, and R-types)."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.org/dc/terms/identifier | "doi:10.1124/jpet.105.087023"xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.org/dc/terms/identifier | "doi:10.1124/jpet.105.087023"xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Cordeiro M.N."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Cordeiro M.N."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Garcia E."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Garcia E."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Kushmerick C."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Kushmerick C."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Snutch T.P."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Snutch T.P."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Gomez M.V."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Gomez M.V."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Stea A."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Stea A."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Hildebrand M.E."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Hildebrand M.E."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Vieira L.B."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/author | "Vieira L.B."xsd:string |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15933156 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |