| http://purl.uniprot.org/citations/15935757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15935757 | http://www.w3.org/2000/01/rdf-schema#comment | "The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2005.03.023"xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/author | "Frank J."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/author | "Gursky R."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/author | "Ehrenberg M."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/author | "Zavialov A."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/author | "Allen G.S."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/pages | "703-712"xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/title | "The cryo-EM structure of a translation initiation complex from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://purl.uniprot.org/core/volume | "121"xsd:string |
| http://purl.uniprot.org/citations/15935757 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15935757 |
| http://purl.uniprot.org/citations/15935757 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15935757 |
| http://purl.uniprot.org/uniprot/#_P0A705-mappedCitation-15935757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15935757 |
| http://purl.uniprot.org/uniprot/#_P69222-mappedCitation-15935757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15935757 |
| http://purl.uniprot.org/uniprot/P69222 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15935757 |
| http://purl.uniprot.org/uniprot/P0A705 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15935757 |