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http://purl.uniprot.org/citations/15950652http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15950652http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To assess the interaction of human proacrosin/acrosin with mannose residues coupled to a protein backbone.

Design

Prospective study.

Setting

Basic research laboratory.

Patient(s)

Recombinant proteins derived from human proacrosin (Rec-40, Rec-30, Rec-20, Rec-10, and Rec-6) and bovine serum albumin (BSA)-mannose as ligand.

Intervention(s)

In vitro binding assay developed to assess proacrosin/acrosin-BSA-mannose interaction.

Main outcome measure(s)

Proacrosin/acrosin binding to BSA-mannose; estimation of binding affinity.

Result(s)

All recombinant proteins of acrosin but Rec-6 (residues 1-59 of proacrosin) specifically bound to BSA-mannose. Rec-40 (proacrosin) showed the highest binding affinity (dissociation constant K(d), 162 nM), followed by N-terminal fragments Rec-30 (248 nM), Rec-20 (359 nM), and Rec-10 (402 nM). A significant decrease in binding activity was observed when acrosin proteins were treated with denaturing agents such as urea or heat. The beta-mercaptoethanol treatment produced a 39% decrease on Rec-30 binding to BSA-mannose; in contrast, no effect was observed with Rec-40, suggesting the presence of at least two types of mannose-binding sites.

Conclusion(s)

[1] Proacrosin interacts with mannose residues through binding sites located at both the N- and C-terminal portion of the protein, [2] the full-length protein is required for maximal BSA-mannose binding, and [3] binding sites are stabilized by noncovalent bonds and by disulfide linkages."xsd:string
http://purl.uniprot.org/citations/15950652http://purl.org/dc/terms/identifier"doi:10.1016/j.fertnstert.2004.12.043"xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/author"Furlong L.I."xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/author"Vazquez-Levin M.H."xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/author"Veaute C."xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/name"Fertil Steril"xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/pages"1791-1796"xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/title"Binding of recombinant human proacrosin/acrosin to zona pellucida glycoproteins. II. Participation of mannose residues in the interaction."xsd:string
http://purl.uniprot.org/citations/15950652http://purl.uniprot.org/core/volume"83"xsd:string
http://purl.uniprot.org/citations/15950652http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15950652
http://purl.uniprot.org/citations/15950652http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15950652
http://purl.uniprot.org/uniprot/P10323#attribution-36A903660ABF460996A7414C4790E353http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/15950652