| http://purl.uniprot.org/citations/15967462 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15967462 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15967462 | http://www.w3.org/2000/01/rdf-schema#comment | "MURF-1, MURF-2 and MURF-3 are a specific class of RING finger proteins that are expressed in striated muscle tissues. MURF-1 has been suggested to act as an ubiquitin ligase, thereby controlling proteasome-dependent degradation of muscle proteins. Here, we performed yeast two-hybrid (YTH) screens of skeletal muscle cDNA libraries with MURF-1 baits to identify potential myocellular targets of MURF-1-dependent ubiquitination. This identified eight myofibrillar proteins as binding partners of MURF-1: titin, nebulin, the nebulin-related protein NRAP, troponin-I (TnI), troponin-T (TnT), myosin light chain 2 (MLC-2), myotilin and T-cap. YTH mating studies with MURF-1,2,3 baits indicated that these eight myofibrillar proteins are all targeted redundantly by both MURF-1 and MURF-2. Western blot studies on cardiac tissues from wild-type and MURF-1-deficient mice suggested that titin and nebulin were ubiquitinated at similar levels, and MLC-2 and TnI at reduced levels in MURF-1 KO mice. Mapping of the TnI and titin binding sites on MURF-1 peptide scans demonstrated their binding to motifs highly conserved between MURF-1 and MURF-2. Our data are consistent with a model in which MURF-1 and MURF-2 together target a specific set of myofibrillar proteins redundantly, most likely to control their ubiquitination-dependent degradation. Finally, our YTH screens identified the interaction of MURF-1 with 11 enzymes required for ATP/energy production in muscle including the mitochondrial ATP synthase and cytoplasmic creatine kinase. These data raise the possibility that MURF-1 may coordinately regulate the energy metabolism of mitochondrial and cytoplasmic compartments."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2005.05.021"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2005.05.021"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Granzier H."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Granzier H."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Labeit S."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Labeit S."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Witt C.C."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Witt C.C."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Witt S.H."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/author | "Witt S.H."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/pages | "713-722"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/pages | "713-722"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/title | "MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitination."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/title | "MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitination."xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/volume | "350"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://purl.uniprot.org/core/volume | "350"xsd:string |
| http://purl.uniprot.org/citations/15967462 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15967462 |
| http://purl.uniprot.org/citations/15967462 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15967462 |