| http://purl.uniprot.org/citations/15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15983206 | http://www.w3.org/2000/01/rdf-schema#comment | "Type 1 diabetes is thought to result from the destruction of beta-cells by autoantigen-specific T-cells. Observations in the NOD mouse model suggest that CD8+ cytotoxic T-cells play an essential role in both the initial triggering of insulitis and its destructive phase. However, little is known about the epitopes derived from human beta-cell autoantigens and presented by HLA class I molecules. We used a novel reverse immunology approach to identify HLA-A2-restricted, naturally processed epitopes derived from proinsulin, an autoantigen likely to play an important role in the pathogenesis of type 1 diabetes. Recombinant human proinsulin was digested with purified proteasome complexes to establish an inventory of potential COOH-terminals of HLA class I-presented epitopes. Cleavage data were then combined with epitope predictions based on the SYFPEITHI and BIMAS algorithms to select 10 candidate epitopes; 7 of these, including 3 with a sequence identical to murine proinsulin, were immunogenic in HLA-A2 transgenic mice. Moreover, six of six tested peptides were processed and presented by proinsulin-expressing cells. These results demonstrate the power of reverse immunology approaches. Moreover, the novel epitopes may be of significant interest in monitoring autoreactive T-cells in type 1 diabetes."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.org/dc/terms/identifier | "doi:10.2337/diabetes.54.7.2053"xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "van Endert P.M."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Lemonnier F.A."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Kratzer R."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Greer F."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Lindo V."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Niedermann G."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Garcia-Pons F."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/author | "Hassainya Y."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/name | "Diabetes"xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/pages | "2053-2059"xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/title | "Identification of naturally processed HLA-A2--restricted proinsulin epitopes by reverse immunology."xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://purl.uniprot.org/core/volume | "54"xsd:string |
| http://purl.uniprot.org/citations/15983206 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15983206 |
| http://purl.uniprot.org/citations/15983206 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C548-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C551-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N3-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N4-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A0G2R0N5-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A076JXB7-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |
| http://purl.uniprot.org/uniprot/#_A0A076L0P9-mappedCitation-15983206 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15983206 |