| http://purl.uniprot.org/citations/15987768 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15987768 | http://www.w3.org/2000/01/rdf-schema#comment | "Vascular smooth muscle cell (VSMC) phenotypic modulation is a key factor in vascular pathology. We have investigated the role of Notch receptor signaling in controlling human vascular smooth muscle cell (hVSMC) differentiation in vitro and established a role for cyclic strain-induced changes in Notch signaling in promoting this phenotypic response. The expression of alpha-actin, calponin, myosin, and smoothelin was examined by performing immunocytochemistry, Western blot analysis, and quantitative real-time PCR in hVSMCs cultured under static conditions after forced overexpression of constitutively active Notch 1 and 3 receptors, inhibition of endogenous Cp-binding factor 1 (CBF-1)/recombination signal sequence-binding protein-Jkappa (RBP-Jkappa) signaling, and exposure to cyclic strain using a Flexercell Tension Plus unit. Overexpression of constitutively active Notch intracellular (IC) receptors (Notch 1 IC and Notch 3 IC) resulted in a significant downregulation of alpha-actin, calponin, myosin, and smoothelin expression, an effect that was significantly attenuated after inhibition of Notch-mediated, CBF-1/RBP-Jkappa-dependent signaling by coexpression of RPMS-1 (Epstein-Barr virus-encoded gene product) and selective knockdown of basic helix-loop-helix factors [hairy enhancer of split (HES) gene and Hes-related transcription (Hrt) factors Hrt-1, Hrt-2, and Hrt-3] using targeted small interfering RNA. Cells cultured under conditions of defined equibiaxial cyclic strain (10% strain, 60 cycles/min, 24 h) exhibited a significant reduction in Notch 1 IC and Notch 3 IC expression concomitant with a significant increase in VSMC differentiation marker expression. Moreover, this cyclic strain-induced increase was further enhanced after inhibition of CBF-1/RBP-Jkappa-dependent signaling with RPMS-1. These findings suggest that Notch promotes changes in hVSMC phenotype via activation of CBF-1/RBP-Jkappa-dependent pathways in vitro and contributes to the phenotypic response of VSMCs to cyclic strain-induced changes in VSMC differentiation."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00198.2005"xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Morrow D."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Walls D."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Sweeney C."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Murphy R."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Guha S."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Cummins P.M."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Scheller A."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Cahill P.A."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Redmond E.M."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/author | "Birney Y.A."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/name | "Am J Physiol Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/pages | "C1188-96"xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/title | "Notch-mediated CBF-1/RBP-J{kappa}-dependent regulation of human vascular smooth muscle cell phenotype in vitro."xsd:string |
| http://purl.uniprot.org/citations/15987768 | http://purl.uniprot.org/core/volume | "289"xsd:string |
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