| http://purl.uniprot.org/citations/16042391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16042391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16042391 | http://www.w3.org/2000/01/rdf-schema#comment | "Cysteine-rich secretory proteins (CRISPs) are widespread in snake venoms. Some members of these CRISPs recently have been found to block L-type Ca(2+) channels or cyclic nucleotide-gated ion (CNG) channels. Here, natrin purified from Naja atra venom, a member of the CRISP family, can induce a further contractile response in the endothelium-denuded thoracic aorta of mouse which has been contracted by a high-K(+) solution. Further experiments show it can block the high-conductance calcium-activated potassium (BK(Ca)) channel in a concentration-dependent manner with an IC(50) of 34.4 nM and a Hill coefficient of 1.02, which suggests that only a single natrin molecule is required to bind an ion channel to block BK(Ca) current. The crystal structure of natrin displaying two domains in tandem shows its cysteine-rich domain (CRD) has relatively independent flexibility, especially for the C-terminal long loop (loop I) of CRD to participate in the interface of two domains. On the basis of previous studies of CNG channel and L-Ca(2+) channel blockers, and the sequence and structural comparison of natrin and stecrisp, the deviation of the vital loop I of CRD is suggested to contribute to different effects of some CRISPs in protein-protein interaction."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi050614m"xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi050614m"xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Guo M."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Guo M."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Liu Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Huang Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Huang Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Hao Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Hao Q."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Lou X."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Lou X."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Niu L."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Niu L."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Shen B."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Shen B."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Teng M."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Teng M."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Duan Y."xsd:string |
| http://purl.uniprot.org/citations/16042391 | http://purl.uniprot.org/core/author | "Duan Y."xsd:string |