| http://purl.uniprot.org/citations/16052286 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16052286 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16052286 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently determined the ORF1 cleavage map of Mc10, a human sapovirus (SaV) strain, as follows: NH2-p11-p28-p35(NTPase)-p32-p14(VPg)-p70(Pro-Pol)-p60(VP1)-COOH. This cleavage was dependent on the viral encoded 3C-like protease. To identify the cleavage site of SaV ORF1, putative p70 (Pro-Pol) and p14-p70 (VPg-Pro-Pol) were expressed as N-terminal GST and C-terminal 6 x His-tag fusion proteins in Escherichia coli, and the expressed products were analyzed by SDS-PAGE and Western blotting. Our results indicated that the efficient proteolytic cleavage occurred between p14 (VPg) and p70 (Pro-Pol), and N-terminal amino acid sequencing revealed that the cleavage site was between E(1055) and A(1056). In contrast, the p70 (Pro-Pol) was not further cleaved. We also found that SaV protease cleaved the Q/G site within the rhinovirus 3C protease recognition site. Site-directed mutagenesis in a conserved GDCG motif of the protease completely abolished these proteolytic activities. This is the first report to identify the cleavage site of the SaV ORF1 polyprotein."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00705-005-0591-0"xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00705-005-0591-0"xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Miyamura T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Miyamura T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Katayama K."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Katayama K."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Ogawa S."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Ogawa S."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Takeda N."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Takeda N."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Kageyama T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Kageyama T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Oka T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Oka T."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Hansman G.S."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/author | "Hansman G.S."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/name | "Arch. Virol."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/name | "Arch. Virol."xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/pages | "2539-2548"xsd:string |
| http://purl.uniprot.org/citations/16052286 | http://purl.uniprot.org/core/pages | "2539-2548"xsd:string |