| http://purl.uniprot.org/citations/16055722 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16055722 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16055722 | http://www.w3.org/2000/01/rdf-schema#comment | "A subset of proteins targeted by the N-end rule pathway bear degradation signals called N-degrons, whose determinants include destabilizing N-terminal residues. Our previous work identified mouse UBR1 and UBR2 as E3 ubiquitin ligases that recognize N-degrons. Such E3s are called N-recognins. We report here that while double-mutant UBR1(-/-) UBR2(-/-) mice die as early embryos, the rescued UBR1(-/-) UBR2(-/-) fibroblasts still retain the N-end rule pathway, albeit of lower activity than that of wild-type fibroblasts. An affinity assay for proteins that bind to destabilizing N-terminal residues has identified, in addition to UBR1 and UBR2, a huge (570 kDa) mouse protein, termed UBR4, and also the 300-kDa UBR5, a previously characterized mammalian E3 known as EDD/hHYD. UBR1, UBR2, UBR4, and UBR5 shared a approximately 70-amino-acid zinc finger-like domain termed the UBR box. The mammalian genome encodes at least seven UBR box-containing proteins, which we propose to call UBR1 to UBR7. UBR1(-/-) UBR2(-/-) fibroblasts that have been made deficient in UBR4 as well (through RNA interference) were significantly impaired in the degradation of N-end rule substrates such as the Sindbis virus RNA polymerase nsP4 (bearing N-terminal Tyr) and the human immunodeficiency virus type 1 integrase (bearing N-terminal Phe). Our results establish the UBR box family as a unique class of E3 proteins that recognize N-degrons or structurally related determinants for ubiquitin-dependent proteolysis and perhaps other processes as well."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.16.7120-7136.2005"xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.25.16.7120-7136.2005"xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Iwamatsu A."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Iwamatsu A."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Varshavsky A."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Varshavsky A."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Lee M.J."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Lee M.J."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Kwon Y.T."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Kwon Y.T."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Mulder L.C.F."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Mulder L.C.F."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Davydov I.V."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Davydov I.V."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Tasaki T."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Tasaki T."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Muesing M."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/author | "Muesing M."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/16055722 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |